Switching the activity of Taq polymerase using clamp-like triplex aptamer structure
نویسندگان
چکیده
منابع مشابه
DNA sequencing using Taq polymerase.
Three DNA polymerases, namely E.coli DNA polymerase 1 (Klenow), reverse transcriptase and T7 DNA polymerase (sequenase), are commonly used for DNA sequencing by the chain termination method of Sanger and colleagues [1). However, the secondary structure of the DNA template can impede the progress of all three polymerases. I have developed a novel procedure in which the thermostable polymerase of...
متن کاملRapid purification of high-activity Taq DNA polymerase.
The method described here is derived from that of Engelke et al. (1), and uses the same cloned form of Ther7nus aquaticus (Taq) DNA polymerase to produce this enzyme in E. coli. The modified purification method described here is quite simple, however it is important to note that factors such as the bacterial strain used, induction time and protein concentration during isolation have been opfimi...
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15 صفحه اولStructure and Mechanism of the DNA Polymerase Processivity Clamp Loader
The replication of a genome is a fundamental biological process that every organism must perform in order to transmit its genetic makeup to its progeny. We have been studying the molecular machines that are used to rapidly and accurately replicate the DNA genome of bacteria. In addition to the polymerase (the actual copying machine), organisms have a set of assemblies that enable the polymerase...
متن کاملSimplified template preparation and improved direct sequencing using Taq polymerase.
A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2020
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkaa581